Hyndman L, Lemoine JL, Huang L, Porteous DJ, Boyd AC, Nan X
Journal Of Controlled Release : Official Journal Of The Controlled Release Society
J Control Release. 2004 Oct 19;99(3):435-44.
The protein transduction domain (PTD) of the HIV-1 Tat protein can facilitate the cellular and nuclear uptake of macromolecular particles. Here, we demonstrate that incorporation without covalent linkage of a 17-amino acid PTD peptide into gene delivery lipoplexes improves gene transfer. Tat/Liposome/DNA (TLD) transfection, as evaluated by Fluorescence Activated Cell Scan analysis of a Green Fluorescence Protein expression plasmid, enabled transfection of highly recalcitrant primary cells in the form of air/liquid interface cultures of sheep tracheal epithelium. Treatment with chloroquine increased, and incubation at low temperature decreased, TLD transfection, suggesting that the endocytosis uptake pathway is involved.
Go Back to Publications View on Pubmed (15451601) View on Google Scholar