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A novel mixing device for the reproducible generation of nonviral gene therapy formulations.

Davies LA, Nunez-Alonso GA, Hebel HL, Scheule RK, Cheng SH, Hyde SC, Gill DR

BioTechniques

Biotechniques. 2010 Sep;49(3):666-8. doi: 10.2144/000113498.

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Nonviral gene therapy utilizing plasmid DNA (pDNA) complexed with cationic lipids (lipoplexes) or cationic polymers (polyplexes) has demonstrated considerable potential for the treatment of a variety of diseases.

However, progress toward clinical application is often delayed by the lack of reliable and scalable mixing of components sufficient to guarantee consistent performance in vivo.

Attempts to improve and standardize mixing have been limited by the sensitivity of pDNA to shear-related degradation. Here we describe a simple pneumatic mixing device that enables the rapid and reproducible production of large volumes of nonviral gene therapy formulations and demonstrate its suitability for use with shear-sensitive pDNA.

 

A novel mixing device

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Other Publications by these authors
Aerosol delivery of DNA/liposomes to the lung for cystic fibrosis gene therapy.
Davies LA et al. Human Gene Therapy. Clinical Development (2014) The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes.
Davies LA et al. Biomaterials (2012) The significance of plasmid DNA preparations contaminated with bacterial genomic DNA on inflammatory responses following delivery of lipoplexes to the murine lung.
Bazzani RP et al. Biomaterials (2011) Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes.
Davies LA et al. Molecular Therapy : The Journal Of The American Society Of Gene Therapy (2008) Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice.
Davies LA et al. Molecular Therapy : The Journal Of The American Society Of Gene Therapy (2008) Identification of transfected cell types following non-viral gene transfer to the murine lung.
Davies LA et al. The Journal Of Gene Medicine (2007)