Cutting Fragments out of an Agarose Gel Under UV (2015): When making novel plasmid DNA constructs, we have to cut DNA fragments out of agarose gels. DNA fragments separate by size on the gel and so can be identified easily when exposed to UV.
Schematic diagram of the large human airways.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Large scale lentivirus production in suspension culture.
A CFTR Western blot, to confirm protein production in cell culture.
Pellets of DNA following precipitation.
Purifying mRNA from tissue samples.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Proposed 3D model of the CFTR protein.
A cake that only some of us got to enjoy!
DNA fragments being cut from an agarose gel exposed to UV.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.